TABLE 1.
Results of testing of different specimens with real-time PCR assays based on the amplification of parts of the P1 gene and the repetitive element repMp1 of M. pneumoniae
| Sample | Descriptiona | M. pneumoniae strain | Real-time PCR | CP ± SDb (n = 8) |
|---|---|---|---|---|
| Purified plasmid pET-30 (containing P1 sequence) | 102 GE/5 μl | M129 (subtype 1) | P1 | 36.45 ± 0.37 (1.0) |
| 104 GE/5 μl | 29.20 ± 0.08 (0.3) | |||
| 106 GE/5 μl | 22.21 ± 0.07 (0.3) | |||
| Purified plasmid pET-30 (containing repMp1 sequence) | 102 GE/5 μl | M129 (subtype 1) | RepMp1 | 35.19 ± 0.30 (0.8) |
| 104 GE/5 μl | 28.43 ± 0.14 (0.5) | |||
| 106 GE/5 μl | 21.55 ± 0.04 (0.2) | |||
| Extracted DNA from bouillon cultures | 0.2 CFU/5 μl | M129 (subtype 1) | P1 | NEc (CP > 42) |
| (0.4 fg of DNA/5 μl) | RepMp1 | 34.16 ± 0.22 (0.6) | ||
| 1.9 CFU/5 μl | P1 | 40.56 ± 1.25 (3.1) | ||
| (4.2 fg of DNA/5 μl) | RepMp1 | 33.64 ± 0.31 (0.9) | ||
| 19.0 CFU/5 μl | P1 | 36.44 ± 0.41 (1.1) | ||
| (42.2 fg of DNA/5 μl) | RepMp1 | 31.15 ± 0.48 (0.6) | ||
| Extracted DNA from bouillon cultures | FH (subtype 2) | P1 | 29.98 ± 0.08 (0.3) | |
| RepMp1 | 26.02 ± 0.06 (0.2) | |||
| 4817 (variant 1) | P1 | 30.95 ± 0.10 (0.3) | ||
| RepMp1 | 26.05 ± 0.09 (0.3) | |||
| ST (variant 2) | P1 | 29.58 ± 0.10 (0.3) | ||
| RepMp1 | 24.70 ± 0.04 (0.2) |
a
GE, genome equivalent.
b
The arithmetic mean of crossing points ± the standard deviation is indicated; the coefficient of variation (%) is given in parentheses. CP, crossing point.
c
NE, not evaluable.