TABLE 2.
Comparison of the results (arithmetic mean of three replicates) of the P1- and repMp1-based real-time PCR in DNA extracts of respiratory tract samples (bronchoalveolar lavage fluids and nasopharyngeal and pharyngeal swabs) of pneumonia patients
| M. pneumoniae straina | Sample | Genome equivalents/reaction ± SD
|
|
|---|---|---|---|
| Real-time PCR (P1 based) | Real-time PCR (RepMp1 based) | ||
| Subtype 1 | CAP-1 | 7.3 × 101 ± 4.0 × 101 | 3.4 × 103 ± 4.5 × 101 |
| CAP-2 | 1.3 × 102 ± 3.2 × 101 | 7.1 × 103 ± 2.6 × 102 | |
| CAP-3 | 9.8 × 103 ± 1.2 × 103 | 1.3 × 105 ± 4.7 × 102 | |
| CAP-4 | 5.0 × 104 ± 1.8 × 103 | 7.4 × 105 ± 1.6 × 104 | |
| CAP-5 | 7.3 × 103 ± 5.2 × 102 | 1.4 × 105 ± 2.1 × 103 | |
| CAP-6 | 1.4 × 104 ± 2.1 × 103 | 2.5 × 105 ± 5.4 × 104 | |
| CAP-7 | 1.8 × 103 ± 4.3 × 101 | 2.0 × 104 ± 1.0 × 104 | |
| CAP-8 | 1.0 × 104 ± 5.8 × 102 | 2.0 × 105 ± 1.3 × 104 | |
| CAP-9 | 2.3 × 105 ± 2.0 × 104 | 1.1 × 107 ± 3.0 × 106 | |
| CAP-10 | 2.7 × 103 ± 1.8 × 102 | 2.0 × 104 ± 4.0 × 103 | |
| CAP-11 | 2.0 × 106 ± 5.2 × 105 | 7.7 × 107 ± 2.0 × 106 | |
| CAP-12 | 4.2 × 103 ± 1.7 × 103 | 1.2 × 104 ± 2.1 × 103 | |
| Subtype 2 | CAP-13 | 4.4 × 102 ± 1.0 × 101 | 1.9 × 103 ± 1.1 × 102 |
| CAP-14 | 8.0 × 104 ± 2.8 × 103 | 9.8 × 105 ± 1.0 × 104 | |
| CAP-15 | 7.0 × 102 ± 2.1 × 101 | 1.2 × 104 ± 8.5 × 102 | |
| CAP-16 | 4.0 × 103 ± 6.8 × 101 | 6.9 × 104 ± 5.2 × 103 | |
| CAP-17 | 2.7 × 103 ± 6.5 × 101 | 6.2 × 104 ± 1.4 × 103 | |
| CAP-18 | 3.9 × 103 ± 3.5 × 102 | 4.9 × 104 ± 1.2 × 104 | |
| Variant 2 | CAP-19 | 4.0 × 103 ± 1.2 × 102 | 1.2 × 105 ± 7.6 × 102 |
| CAP-20 | 9.5 × 104 ± 5.4 × 103 | 2.4 × 106 ± 9.8 × 104 | |
| CAP-21 | 5.0 × 104 ± 8.0 × 103 | 1.3 × 106 ± 8.3 × 104 | |
| CAP-22 | 2.7 × 103 ± 3.8 × 102 | 5.6 × 104 ± 1.8 × 103 | |
| CAP-23 | 1.6 × 105 ± 2.6 × 103 | 2.2 × 106 ± 1.2 × 105 | |
| CAP-24 | 1.8 × 105 ± 3.0 × 103 | 4.5 × 106 ± 1.0 × 106 | |
| CAP-25 | 2.2 × 105 ± 1.6 × 104 | 4.1 × 106 ± 3.4 × 105 | |
a
Tested M. pneumoniae positive and subtyped according to the method of Dumke et al. (5).