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. 2007 Jun 13;45(8):2521–2528. doi: 10.1128/JCM.02117-06

FIG. 2.

FIG. 2.

Sensitivities of Plasmodium genus- and species-specific real-time LAMP assays performed with serial dilutions of plasmid DNA (106 copies to 1 copy per reaction) containing an 18S rRNA gene. (A) Amplification with a Plasmodium genus-specific primer set. One representative result of four replicates is shown. Samples contained a plasmid harboring the P. falciparum 18S rRNA gene. (B) Plot of the mean threshold time of the Plasmodium genus-specific LAMP. The error bars represent the standard errors of the mean values from four replicates. The plot of the mean threshold time against the log of the input DNA fit a linear function (r2 = 0.96). (C) Amplification with the P. falciparum species-specific primer set. One representative result of four replicates tested with the plasmid harboring the P. falciparum 18S rRNA gene is shown. (D) Plot of the mean threshold time of the P. falciparum species-specific LAMP from four replicates, which fit a linear function (r2 = 0.91). (E) Amplification with a P. vivax species-specific primer set. One representative result of four replicates tested with plasmid harboring P. vivax 18S rRNA gene is shown. (F) Plot of the mean threshold time of the P. vivax species-specific LAMP from four replicates, which fit a linear function (r2 = 0.95). (G) Amplification with a P. malariae species-specific primer set. One representative result of four replicates tested with a plasmid harboring the P. malariae 18S rRNA gene is shown. (H) Plot of the mean threshold time of the P. malariae species-specific LAMP from four replicates, which fit a linear function (r2 = 0.84). (I) Amplification with a P. ovale species-specific primer set. One representative result of four replicates tested with a plasmid harboring the P. ovale 18S rRNA gene is shown. (J) Plot of the mean threshold time of the P. ovale species-specific LAMP from four replicates, which fit a linear function (r2 = 0.90). Genus, genus Plasmodium LAMP; Pf, P. falciparum LAMP; Pv, P. vivax LAMP; Pm, P. malariae LAMP; Po, P. ovale LAMP; O.D., optical density.