TABLE 2.
Detailed comparison of microscopy, nested PCR, and LAMP for malaria parasite detection and species identification
| Parasite(s) detected by each method (no. of samples)a
| ||
|---|---|---|
| Microscopy | Nested PCR | LAMPb |
| P. falciparum (12)c | P. falciparum (12) | P. falciparum (12) |
| P. falciparum + P. vivax (5) | P. falciparum + P. vivax (4), P. vivax (1) | P. falciparum + P. vivax (4), P. vivax (1) |
| P. vivax (34) | P. vivax (30), P. vivax (1), P. vivax (1), P. ovale (2) | P. vivax (30), P. vivax + P. falciparum (1), negative (1), P. ovale (2) |
| P. malariae (12) | P. malariae (8), P. malariae + P. vivax (1), P. ovale (1), P. malariae (1), negative (1) | P. malariae (8), P. malariae + P. vivax (1), P. ovale (1), Plasmodium spp.d (1), P. malariae (1) |
| P. ovale (5) | P. ovale (5) | P. ovale (5) |
| Negative (53) | Negative (50), P. falciparum (1), P. vivax (2) | Negative (50), P. falciparum (1), P. vivax (2) |
a
Results that were nonconcordant between nested PCR and LAMP are underlined.
b
LAMP assays were run twice; in all cases the two experiments gave the same results.
c
Each row provides the results obtained with identical DNA samples.
d
Positive for the genus Plasmodium by LAMP but negative by all four species-specific LAMPs.