FIG. 1.

Transcription map of AAV5. (A) The AAV5 genome is depicted at the bottom of the figure and includes the locations of the viral promoters (Inr, P7, P19, and P41), the small intron donor (D), acceptors (A1 and A2), the termination site for the Rep proteins, the internal polyadenylation site (pA)p, and the inverted terminal repeats (ITRs). The major transcript classes and the proteins that they encode are shown above. The different ORFs that are used are shown in different shading patterns. It is not clear whether a portion of Inr-initiated RNA is spliced or not; this is indicated by a dashed line. (B) The AAV5 intron is shown in more detail, with sequences of the donor, poly(A) signal, and acceptor. The DH probes used for subsequent RPAs are also shown.