Skip to main content
. 2007 May 16;81(15):7974–7984. doi: 10.1128/JVI.00714-07

FIG. 2.

FIG. 2.

Overexpression of U1-70K decreased polyadenylation of AAV5 RNA at (pA)p. (A) RPA, using the DHA2CAA probe, of RNA isolated from 293 cells transfected with P7J1650CapA2CAA, together with either the control plasmid SK (pBluescript, Stratagene) (lane 1) or plasmids expressing the U1A, U1C, or U1-70K proteins (pCIHAU1A [lane 2], pCIHAU1C [lane 3], or pCIHAU1-70K [lane 4], respectively). Bands representing RNAs that either read through or are polyadenylated at (pA)p are designated (pA)d or (pA)p, respectively. Quantifications of the ratios of P7-generated RNA at (pA)p to total P7-generated RNA [(pA)p+(pA)d] are shown as percent (pA)p and are averages of the results for at least three individual experiments with standard deviations. (B) Map of plasmid P7J1650CapA2CAA and the location of the DHA2CAA probe. (C) Immunoblot analysis, using an anti-HA antibody, showing similar levels of expression of the various U1 proteins.