FIG. 7.

Northern blots of VZV IE, putative early, and late transcripts in MeWo cells in the presence (+) or absence (−) of IFN-α. (A) MeWo cells untreated or pretreated with human IFN-α (1,000 IU/ml) were infected with ROka, ROka63D, or ROka61D. Total RNA was isolated 2 days after infection, and Northern blots were performed using radiolabeled DNA probes specific for VZV IE62, TK, gE, and cellular β-actin. (B) Bands were quantified using a densitometer, and each band was normalized to the area of the corresponding β-actin band. Reduction (n-fold) in mRNA expression due to IFN-α treatment was calculated as the ratio of densitometry values from IFN-α-untreated cells to that from IFN-α-treated cells. The data are representative of one of two independent experiments.