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. 2007 May 23;81(15):8041–8049. doi: 10.1128/JVI.00068-07

FIG. 2.

FIG. 2.

Expression analysis of CCR5Δ32 protein in infected and uninfected CCR5−/− individuals. (A) Immunodetection of native CCR5Δ32 protein in PBMCs isolated from individuals homozygous (−/−) and heterozygous (+/−) for the CCR5Δ32 mutant allele. The Western blot was probed with antibodies generated against the novel frameshift amino acids that specifically detect CCR5Δ32 protein. (B) The blot in panel A was stripped and reprobed with monoclonal antibodies to GAPDH to control for gel loading. (C) Immunodetection (same procedure as described for panel A) of native CCR5Δ32 protein in 11 CCR5−/− and 1 (+/−) PBMC samples obtained from the MACS. (D) The blot in panel C was stripped and reprobed with GAPDH antibodies. The numbers above each lane represent MACS sample numbers. PT 2, patient 2.