FIG. 1.
Inactivation of PV with a mouse gastric solution. (A) PV1(M)OM at 2 × 105 PFU/5 μl was incubated at 37°C or 0°C for the periods indicated with or without 45 μl of a gastric solution from PVRTg21 (Tg) or C57BL/6 (non-Tg) mice, and then the titer of virus was determined by a plaque assay. As a control, PV was incubated with 45 μl of saline. Three animals were used per group. (B) PV1(M)OM at 1 × 105 PFU/5 μl was incubated at 37°C or 0°C for the periods indicated with 45 μl of each solution. After the incubation, the titer of virus was determined by a plaque assay. Open bars, samples just after mixing; solid bars, incubation at 37°C for 4 h; shaded bars, incubation at 0°C for 4 h. Asterisks, nondetectable titers. ND, not detected.