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Negative-stain EM images of (A) wt AAV2, (B) AAV2-ala, (C) AAV2-cys, and (D) AAV2-trp that identified the unique staining pattern of the Leu336 mutant virions. The insets are magnified close-ups of the AAV2 virions. Symbols in panels C and D represent the staining pattern/profile of the fivefold mutant virlons.
