FIG. 3.

Localization of capsids and glycoproteins in human and rat neurons infected with HSV SC16 gD-YFP. SK-N-SH neurons (A) or rat dorsal root ganglion neurons (B) were infected with SC16 gD-YFP (1 PFU/cell) for 26 h, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and stained with mouse anti-VP5 MAbs, followed by Texas Red-conjugated donkey anti-mouse immunoglobulin G as previously described (18). (C to E) SK-N-SH neurons were infected with SC16 gD-YFP, fixed, permeabilized, and stained with rabbit anti-gD polyclonal antibodies, followed by Texas Red-conjugated donkey anti-rabbit immunoglobulin G. Panels: C, gD-YFP fusion; D, rabbit anti-gD polyclonal antibody staining; E, merged fluorescence. Scale bars = 4 μm.