FIG. 2.
Analysis of envelope incorporation by V3 mutant chimeric viruses. To ensure equivalent incorporation of the envelope protein in the chimeric viruses, equal TCID50 of replication competent virus was pelleted at 38,000 rpm and subjected to Western blot. (A) 103.5 infectious units of each virus were pelleted and resuspended in 100 μl of SDS lysis buffer. A 10-μl portion of this sample, or 316 infectious units, was diluted serially 1:5 for each NL4-3-V3A1-92RW009 chimeric virus and probed for gp120 (B13 MAb), gp41 (Chessie 8 MAb), and p24 (24-4 MAb). (B) 316 infectious units of each NL4-3-gp120B5-91US056 and NL4-3-gp160Yu-2 V3 crown mutant chimeric virus were probed for p24 and gp120 content.