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. 2007 May 23;81(15):8080–8090. doi: 10.1128/JVI.02727-06

FIG. 5.

FIG. 5.

Replication of SIVagm in CypA-deficient cells is Vif independent. (A) Virus stocks were produced from transiently transfected HeLa cells and used for the infection of PPIA−/− Jurkat cells. Virus production was monitored for 18 days by determining the virus-associated reverse transcriptase activity in the culture supernatants. (B) CsA eliminates the requirement for Vif for SIVagm replication in Jurkat cells. WT or vif-defective SIVagm (left panel) and HIV-1 (right panel) produced in HeLa cells were used to infect Jurkat cells. Infected cells were cultured in the presence of CsA (2.5 μM). Virus production was monitored for 18 days by determining the virus-associated reverse transcriptase activity in the culture supernatants. (C) The infectivity of the viruses at peak virus production shown in panel B was determined by infection of LuSIV indicator cells, as described in the legend to Fig. 1B. The infectivity of the WT virus in untreated cells was defined as 100%. Error bars reflect the standard deviations calculated from triplicate experiments.