FIG. 6.
Role of PKR in the NF-κB activation pathway. PKR+ and PKRkd HeLa cells were transfected with an NF-κB-dependent firefly luciferase reporter plasmid. Transfected cells were mock treated or treated with either 10 ng/ml TNF-α or 100 μg/ml dsRNA and 50 μg/ml DEAE-dextran at 48 h after transfection. (A) TNF-α. Luciferase activity was measured at 4 h after TNF-α treatment (hatched bars) or in cells left untreated (open bars). (B) dsRNA. Luciferase activity was measured in dsRNA mock-treated and treated cells at 4, 6, and 8 h after dsRNA treatment of PKR+ (circles) and PKRkd (squares) HeLa cells. Results shown are the means ± standard deviations determined from a minimum of six independent experiments.