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Single-cycle multiplication of adenovirus in PKR+ and PKRkd HeLa cells. Cells were either mock treated (-) or treated with 1,000 units/ml IFN-α A/D (+) for 24 h and then infected with either wild-type adenovirus (A) or VAI deletion mutant adenovirus (B). At 36 h p.i., cells were harvested and virus yields determined by plaque titration. Based on Student's t test, P was >0.18 between infected cell pairs.
