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. 2007 May 23;81(15):7999–8008. doi: 10.1128/JVI.00348-07

FIG. 2.

FIG. 2.

Internal cleavage of the NS3 protein in the presence of NS4A. (A) Internal cleavage of NS3 in Huh7 cells. Plasmids encoding V5-tagged HCV NS3 and NS4A proteins were cotransfected into Huh7 cells. Cell lysates were harvested 2 days posttransfection for Western blot analysis with anti-V5 antibody. (B) Internal cleavage of NS3 in mouse liver tissue. Plasmids pAdTrack-NS3-V5His and pcDNA-NS4A-V5 were introduced into mouse liver tissue by hydrodynamics injection. Western blot analysis was performed with tissue homogenates prepared at 8 or 24 h after injection as indicated with antibody against the V5 epitope. GFP coexpressed with NS3 from pAdTrack-NS3-V5His was analyzed in parallel as an internal control. (C) Effect of T402A mutation on the internal cleavage activity of NS3. Plasmids encoding V5-tagged HCV proteins as indicated were transfected into Huh7 cells. Cell lysates were harvested 2 days posttransfection for Western blot analysis with anti-V5 antibody.