TABLE 3.
Expression levels of selected genes by real-time RT-PCRa
| Gene product | Fold change by real-time RT-PCR
|
|||||
|---|---|---|---|---|---|---|
| MVA vs mock
|
NYVAC vs mock
|
|||||
| DC 1 | DC 2 | DC 3 | DC 1 | DC 2 | DC 3 | |
| TNF | 36.63 | 25.45 | 44.32 | 2.23 | 4.95 | 4.48 |
| IFN-β | 43.86 | 28.87 | 27.76 | 2.15 | 4.98 | 1.85 |
| ISG15 | 3.82 | 4.62 | 3.81 | 2.15 | 2.27 | 2.26 |
| IL-12 | 7.91 | 5.89 | 7.98 | 2.08 | 1.98 | 1.79 |
| IL-7 | 1.78 | 0.90 | 2.07 | 1.27 | 1.55 | 0.81 |
| IL-6 | 2.01 | 2.36 | 3.03 | 1.16 | 1.41 | 2.54 |
| IFN-γ | 1.45 | 1.82 | 1.4 | 1.19 | 0.98 | 1 |
| NFκ2 | 2.02 | 3.05 | 3.64 | 1.68 | 2.06 | 2 |
| OASL | 27.66 | 37.66 | 26.63 | 2.13 | 1.43 | 0.519 |
| ATF-3 | 1.96 | 2.19 | 1.98 | 3.51 | 3.75 | 3.91 |
| H2AFY | 1.44 | 1.89 | 1.98 | 3.73 | 0.75 | 0.91 |
| ADORAA2 | 0.714 | 1.18 | 3.6 | 1.28 | 1.18 | 0.63 |
a
MDDC from three donors (DC1, DC2, and DC3) were mock, MVA, or NYVAC infected and processed for RT-PCR.