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RBP-Jk is essential for Rta-mediated transactivation of the K-bZIP promoter. MEFs null for RBP-Jk (OT11) were transfected with expression vectors corresponding to Rta (pcDNA3FLc50) and RBP-Jk (pcDNA3.1-RBP-Jk). Transfections were analyzed as described in the legend to Fig. 3A.
