TABLE 2.

Transactivation and stimulation of reactivation by Rta OIDa mutants that are deficient in DNA and/or Oct-1 binding

ORF50/Rta	Oct-1 binding resulta	DNA binding resultb	% Transactivationc	% Reactivationd
WT	+++	+++	100	100
L136A	−	+	35	34
I140A	+	+++	104	35
L143A	+++	++++	104	117
L144A	+	−	55	51
ILL140AAA	−	−	1	1
ADPRins	−	−	1	ND

^aGST pull-down (Fig. 5A, B, and E). WT is set at “+++.”

^bEMSA (Fig. 6). WT is set at “+++.”

^cMaximal value in transient transactivation of K-bZIP promoter in Akata-31 cells (Fig. 7A), expressed as a percentage compared to the WT (set at 100%).

^dInduction of K8.1 expression in BCBL-1 cells (Fig. 10), expressed as a percentage compared to the WT (set at 100%). ND, not done.