Skip to main content
. 2007 May 23;81(16):8412–8420. doi: 10.1128/JVI.00189-07

FIG. 3.

FIG. 3.

mRNA 5′ UTR does not affect geldanamycin-mediated suppression of FHV protein A synthesis. (A) Schematics of pS2FA and derivatives with modified 5′ UTRs. All plasmids contain an MT promoter and a simian virus 40 polyadenylation signal (An). The 5′ UTR of pS2FA contains 204 nucleotides (nt) with an EMCV IRES, whereas pS2FA-Gal L contains 168 nt with the yeast GAL1 leader (Gal L). The 5′ UTR of pS2FA-5′vUTR contains 39 nt with the authentic FHV 5′ UTR sequence (F). ORF, open reading frame. (B) Drosophila S2 cells stably transfected with pS2FA-Gal L or pS2FA-5′vUTR were induced with Cu2+ and labeled with 100 μCi per ml [35S]Met-Cys for 90 min in the presence of vehicle only or decreasing geldanamycin (GA) concentrations (conc.). Radiolabeled protein A was immunoprecipitated with HA-specific antibodies, separated by SDS-PAGE, and analyzed by fluorography. Results are representative of three or four independent experiments, and the quantitative values are the means from those experiments relative to vehicle-only controls.