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Plaque phenotypes of RICO-ΔZ, CBV-RICO, CBV-ΔZ, wt CBV3, and their variants containing the identified amino acid exchanges; all amino acid exchanges affect 3A except [D51G], which occurs in 3C. Individual constructs were derived as described in Materials and Methods, and their plaque phenotype was determined by analyzing cell lysates obtained 48 h after transfection of infectious in vitro transcript genomic RNA. N.D., not done.
