Skip to main content
. 2007 May 30;81(16):8634–8647. doi: 10.1128/JVI.00418-07

FIG. 1.

FIG. 1.

Temporal distribution of N in Vero E6 cells infected with HTNV or transfected with plasmid expressing N. (A) To ensure maximal infection, Vero E6 cells were infected with HTNV at an MOI of 5.0 and were examined for the presence of N at 4, 12, 24, and 72 h with (upper row) or without (lower row) phase contrast. At each of these times, cells were acetone fixed and stained by indirect immunofluorescence for N (red) with rabbit polyclonal anti-N antibody no. 143 as described in Materials and Methods. Scale bar, 20 μm, with 63× objectives (Axiovert 200 microscope). The nucleus was stained with 4′,6′-diamidino-2-phenylindole. (B) Vero E6 cells were transfected with pcHTNVN and examined for the presence of N at 4, 12, 24, and 48 h. At each of these times, cells were acetone fixed and stained by indirect immunofluorescence for N (red) with rabbit polyclonal anti-N antibody no. 143 as described in Materials and Methods. Scale bar, 20 μm, with 63× objectives (Axiovert 200 microscope).