FIG. 4.

Association of HTNV N with membrane fractions. Vero E6 cells were transfected with pcHTNVN, and after 18 h they were subjected to membrane floatation (A) or subcellular fractionation (B). Fractions were subjected to Western blotting and were probed with antibody to CNX, N, or actin. (A) Vero E6 cell extracts containing transiently expressed N were brought to 1.4 M sucrose and centrifuged as described in Materials and Methods. Fractions were collected from the top, and proteins were precipitated. (B) Immunoblot analyses of PNS, soluble supernatant (S), and particulate pellet (P) fractions by following the treatment or no treatment (NT) and centrifugation regimen described in Materials and Methods. TX-100, Triton X-100.