FIG. 8.

Analysis of NSs mRNA transcription termination control. The S-IGR sequences in NS IGR-1 and other mutants are shown. The conserved 3′-CCCGUCGG-5′ and 3′-CCGACGGG-5′ sequences are boxed. The NSs mRNA termination site is denoted by a dotted line with terminal arrows. Svc represents the anti-virus-sense minigenome. The numbers represent the nucleotide positions of the S-IGR from the 3′ end of the virus-sense S segment. The rLuc probe was used for Northern blot analysis (see the legend to Fig. 7). The efficiency of the rLuc mRNA transcriptional termination of each minigenome (relative termination) was determined as described in the legend to Fig. 7.