TABLE 4.

2G12 neutralization sensitivity and epitope sequences

Virus variant	2G12 IC50 (μg/ml)	2G12 epitope stretcha
		295	332	339	386	392
B.1.A6	>12.50	−*	+	−†	+	+
B.1.B5	>12.50	−*	+	−†	+	+
B.1.G11	>12.50	−*	+	−†	+	+
B.2.A12	>12.50	−*	+	−†	+	+
B.2.B7	>12.50	−*	+	−†	+	+
B.2.E6	>12.50	−*	+	−†	+	+
B.2.G11	>12.50	−*	+	−†	+	+
B.3.A12	>12.50	−*	+	−†	+	+
B.3.D7	>12.50	−*	+	−†	+	+
B.3.F3	>12.50	−*	+	−†	+	+
B.3.H5	>12.50	−*	+	−†	+	+
B.4.B1	>12.50	−*	+	−†	+	+
B.5.B8	>12.50	−*	+	−†	+	+
B.7.D11	>12.50	−*	+	−†	+	+
B.7.H8	>12.50	−*	+	−†	+	+
B.11.A8	>12.50	−*	+	+	+	+
B.11.E10	>12.50	−*	+	+	+	+
B.11.G11	>12.50	−*	+	+	+	+
B.13.E4	>12.50	−	+	−	+	−
B.13.G11	>12.50	−*	+	+	+	+
D.1.H7	1.27	+	+	+	+	+
D.1.B8	>12.50	+	+	+	+	+
D.1.G1	0.34	+	+	+	+	+
D.3.A10	>12.50	+	+	+	+	+
D.3.G1	>12.50	+	+	+	+	+
D.7.A5	0.22	+	+	+	+	+
D.7.F8	3.19	+	+	+	+	+
D.7.G3	4.61	+	+	+	+	+
F.2	>12.50	+	+	-	-	+
F.3.F6	>12.50	+	+	-	-	+
F.3.A12	>12.50	+	+	-	-	+
F.3.G9	>12.50	+	+	-	-	+
F.4	>12.50	+	+	-	-	+
F.4.II	>12.50	+	+	-	-	+
F.5.A8	>12.50	+	+	-	-	+
F.5.B2	>12.50	+	+	-	-	+
F.5.G8	>12.50	+	+	-	-	+
F.7.F6	>12.50	+	+	-	-	+
F.7.G11	>12.50	+	+	-	-	+
F.7.G4	>12.50	+	+	-	-	+
F.9.F5	>12.50	+	+	-	-	+
F.9.B7	>12.50	+	+	-	-	+
W.I.3	>12.50	-	+	+	-	+
W.I.6	>12.50	-	+	+	-	+
W.I.16	>12.50	-	+	+	-	+
W.II.1	>12.50	-	+	+	-	+
W.II.4	>12.50	-	+	+	-	+
W.II.5	>12.50	-	+	+	-	+
W.III.2	>12.50	-	+	+	-	+
W.III.6	>12.50	-	+	+	-	+
W.III.7	>12.50	-	+	+	-	+
W.IV.1	>12.50	-	+	+	-	+
W.IV.2	>12.50	-	+	+	-	+
W.IV.4	>12.50	-	+	+	-	+
W.V.E6	0.50	+	+	+	+	+
W.V.E7	0.83	+	+	+	+	+
W.VI.B6	<0.20	+	+	+	+	+
D1.6F1	4.30	+	+	+	+	+
D1.6F3	>12.50	+	+	+	+	+
R1.6F5	>12.50	+	+	+	+	+
R1.6G9	>12.50	+	+	+	+	+
R1.48.E1	0.39	+	+	-	+	+
R1.48C4	0.41	+	+	-	+	+
R1.48D4	0.36	+	+	-	+	+
D2.3A6	>12.50	+	+	+	+	+
D2 3F4	>12.50	+	+	+	+	+
D2.3.D12	9.54	+	+	+	+	+
D2.3C3	3.70	+	+	+	+	+
R2.8A6	>12.50	-	+	+	+	+
R2.8D6	>12.50	-	+	+	+	+
R2.8C6	>12.50	-	+	+	+	+
R2.8D2	>12.50	-	+	+	+	+
R2.8F6	>12.50	-	+	+	+	+
R2.54H8	0.58	+	+	+	+	+
R2.54G11	0.43	+	+	+	-	+
D3.14A5	0.32	+	+	+	+	+
D3 14E3	0.31	+	+	+	+	+
D3.14G1	<0.20	+	+	+	+	+
D3.14B2	0.58	+	+	+	-	+
R3.3A9	>12.50	+	+	+	+	+
R3.3D9	>12.50	+	+	+	+	+
D4.3E9	<0.20	+	+	+	+	+
D4.3H4	<0.20	+	+	+	+	+
D4.5A11	<0.20	+	+	+	+	+
D4.5C8	<0.20	+	+	+	+	+
D4.5F10	<0.20	+	+	+	+	+
D4.5G3	<0.20	+	+	+	+	+
D4.5H9	<0.20	+	+	+	+	+
R4 15A9	>12.50	+	+	+	+	+
R4 15D5	>12.50	+	+	+	+	+
R4 15F10	<0.20	+	+	+	+	+
R4 15F9	>12.50	+	+	+	+	+
R4 15H6	>12.50	+	+	+	+	+
R4 43A11	2.45	+	+	+	+	+
R4 43B12	>12.50	+	+	+	+	+
R4 43E1	>12.50	+	+	+	+	+
PD5.D8	>12.50	+	+	-	+	+
PD5.E5	>12.50	+	+	-	+	+
PD5.E8	>12.50	+	+	-	+	+
PD5.F3	>12.50	+	+	-	+	+
PD5.D6	>12.50	+	+	-	+	+
PD5.F7	>12.50	+	+	-	+	+
PD5.H11	>12.50	+	+	-	+	+
PD5.F2	>12.50	+	+	-	+	+
PD5.G5	>12.50	+	+	-	+	+
PR5.O4.A2	>12.50	+	+	-	+	+
PR5.O4.A3	>12.50	+	+	-	+	+
PR5.O4.A5	>12.50	+	+	-	+	+
PR5.O4.B1	>12.50	+	+	-	+	+
PR5.O4.C7	>12.50	+	+	-	+	+
PR5.O4.D1	>12.50	+	+	-	+	+
PR5.O4.F4	>12.50	+	+	-	+	+
PR5.O8.B6	>12.50	+	+	-	+	+
PR5.O8.C5	>12.50	+	+	-	+	+
PR5.O8.F11	>12.50	+	+	-	+	+
PR5.O8.D4	>12.50	+	+	-	+	+
PD6.A10	>12.50	+	+	-	+	-
PD6.C11	10.40	+	+	-	-	+
PD6.D9	>12.50	+	+	-	+	+
PD6.E3	>12.50	+	+	-	+	+
PD6.F2	>12.50	+	+	-	+	+
PD6.A9	>12.50	+	+	-	-	+
PD6.C10	>12.50	+	+	-	-	+
PD6.D7	8.16	+	+	-	-	+
PD6.E8	>12.50	+	+	-	-	+
PD6.F10	>12.50	+	+	-	-	+
PR6.A10	0.18	+	+	-	+	+
PR6.A2	<0.20	+	+	-	+	+
PR6.A7	1.27	+	+	-	+	+
PR6.E10	<0.20	+	+	-	-	+
PR6.F3	>12.50	+	+	-	-	+
PR6.G8	0.20	+	+	-	-	+
PR6.11.A5	<0.20	+	+	-	+	+
PR6.11.B3	7.20	+	+	-	+	+
PR6.11.C1	0.62	+	+	-	+	+
PR6.12.A4	<0.20	+	+	-	+	+
PR6.12.C6	>12.50	+	+	-	+	+

^aNumbers represent amino acid positions, relative to HXB2; +, presence of PNGS; −, absence of PNGS. *, Presence of a PNGS at position 293; †, presence of a PNGS at position 337.