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Preexpression of nuclear pp71 rescues IE1 gene expression. THP-1 monocytes were mock transduced (lane M) or transduced with rAD-EF1α pp71 (lane 71), rAD-EF1α without a transgene (lane E), rAD-CMV pp71 Did2-3 (lane D2), or rAD-CMV without a transgene (lane C) at 2,000 particles per cell for 18 h. Subsequently, all of the cells were infected with HCMV at an MOI of 3 for 24 h. Lysates were harvested and analyzed by Western blotting. Tub, tubulin.
