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. 2007 Jun 27;81(17):8891–8904. doi: 10.1128/JVI.00614-07

FIG. 3.

FIG. 3.

Reversion to the parental SIVsmE543-3 sequence in SIVsmH635FC-infected macaques. (A) The env V1-V5 region was amplified by RT-PCR from plasma samples from day 10 or 11 (10d/11d), week 20 (20w), and week 40 or 41 (40w/41w). RT-PCR products were cloned into plasmids and sequenced. Reversion to the SIVsmE543-3 sequence at the seven locations which differ between SIVsmE543-3 and SIVsmH635FC in the env V1-V5 region is shown by amino acids. The seven locations of the amino acid substitutions, namely, N158D, P337T, E340K, R348W, D388N, P430S, and D519N, are shown on the left side. Amino acid residues at these locations of the obtained clones are boxed to represent the sampling date and macaques. Clone numbers are shown at the tops of the boxes. Amino acids that reverted to SIVsmE543-3 amino acids are shown by white letters in a black background. Amino acid changes other than the changes that revert to the SIVsmE543-3 sequence are shaded in gray. (B) The kinetics of reversion to the SIVsmE543-3 sequence are shown. The percentage of reversion was calculated using all the clones from the four SIVsmH635FC-infected macaques at day 10 or 11, week 20, and week 40 or 41 shown in panel A. The percentages of reversion at 6 and 10 weeks were calculated using clones from two macaques, H704 and H709.