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. 2007 Jun 20;81(17):9121–9130. doi: 10.1128/JVI.00136-07

FIG. 3.

FIG. 3.

LZ1 differs from wt LMP1 in its distribution in cells. 293 cells were transiently cotransfected with 0.5 to 2 μg of plasmids expressing derivatives of LMP1. Signals from full-length wt LMP1GFP and wt LMP1RFP, LZ1GFP and LZ1RFP (A) and wt LMP1 and LZ1 (B) served as positive controls for colocalization. Wt 1-2TM LMP1 GFP and full-length LMP1 RFP (C) and LZ1 1-2TM GFP and full-length LZ1 RFP (D) did not colocalize. Wt 1-2TM GFP and LZ1 1-2TM RFP (E) and LZ1 1-2TM GFP and wt 1-2TM RFP (F) also failed to colocalize in cells. Similar patterns of localization were found with all of the derivatives when the fluorescent tags were switched. (G and H) Profiles of the intensities of the fluorescent molecules in a cross-section. The white arrows in the merged images indicate the line and direction of the measured intensities. (G) The merged image of cells cotransfected with LZ1 GFP and wt LMP1 RFP (the same image is shown in panel B, first row) is shown as a representative of a colocalized signal. (H) The merged image of a cell cotransfected with LZ1 1-2TM GFP and wt LMP1 1-2TM RFP (the same image is shown in panel F, second row) is shown as a representative of signals that did not colocalize.