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Linear range of responses of the Western blots for GAP-43 and other synaptic proteins. (A) Samples containing 5, 10, 20, and 35 μg of human SPM protein were analyzed by Western blots (see Materials and Methods). (B) Densitometric quantitation of the band intensities corresponding to GAP-43 showed linear responses between 5 and 35 μg of total protein loaded.
