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. 2007 Jun 13;81(17):8953–8966. doi: 10.1128/JVI.00649-07

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Copyright © 2007, American Society for Microbiology

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FIG. 5. — NS5A interacts with MyD88. MyD88-His was coexpressed with Flag-core/E1/E2 or -NS5A (A) or Flag-NS3, -NS3/4A, -NS4B, -NS5A, or -NS5B (B) in 293T cells; immunoprecipitated (IP) with anti-Flag, E1, or E2 antibody; and immunoblotted (IB) with anti-His antibody. (C) Flag-NS5A was coexpressed with MyD88-His, TRAM-His, TIRAP-His, or TRIF-HA in 293T cells and immunoprecipitated with anti-His or -HA antibody. The immunoprecipitates were immunoblotted with anti-Flag antibody. Asterisks indicate nonspecific bands.