FIG. 1.
Pro-35 enhances the replication of R5-tropic HIV-1 in HLT. (A) N-terminal structure and amino acid sequence of Vpr from HIV-1NL4-3. Proline residues in positions 5, 10, 14, and 35, as well as p6 binding regions, are indicated (4, 16). (B) Representative replication profiles of R5-tropic HIV-1 and Vpr mutants in HLT ex vivo. RT, reverse transcriptase. (C and D) For average virus production, tissue samples were inoculated with X4-tropic and R5-tropic viruses, respectively, and cumulative virus release by 18 to 21 tissue blocks over 15 days was measured. Bars show mean virus release relative to that for the wt virus. Error bars, SEMs.