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Binding of full-length T-ag to DNA measured by fluorescence anisotropy. Binding isotherms of full-length T-ag (squares) by use of a 24-bp probe containing (filled symbols) or lacking (open symbols) a single TBS and performed in the absence (A) or presence (B) of 4 mM AMP-PNP and 7 mM MgCl2. In panel A, binding isotherms generated with the N260 protein (circles) under the same conditions are shown in gray for comparison. Binding isotherms were performed in duplicate.
