FIG. 7.
Systematic mutagenesis of P3. (A) Nucleotide sequence of the oligonucleotide containing P3 (indicated by an arrow). Nucleotides shown in bold were systematically changed for all three other nucleotides. The apparent Ki values associated with each change are shown in the bar graph. Apparent Ki and standard deviations were calculated from the IC50 of each competitor DNA obtained in triplicate in assays performed at 250 nM of N260 (filled bars) or OBD (open bars) and 15 nM of one TBS probe. (B) Consensus binding site determined from the data in panel A. (C) Location of two putative novel TBSs (P5 and P6) in site II suggested by the consensus sequence obtained as described above.