FIG. 1.
Maps of virus mutants and plasmids. (A) The PrV genome, consisting of long (UL) and short (US) unique regions and inverted repeat sequences (IR, TR), is depicted (B) Enlarged sections show the viral ORFs (pointed rectangles) within the analyzed genome regions. Recombinant viruses were generated by mutagenesis in E. coli of an infectious full-length clone of the PrV-Ka genome, which led to insertion of resistance genes (KanR/TetR) or FRT. In PrV-UL3.5G, the EGFP ORF was fused in-frame to UL3.5, which also affected the 3′ end (shaded) of the overlapping UL3 gene. (C) Plasmids used for yeast two-hybrid studies contained UL48 of PrV, and complete or truncated UL3.5 genes of PrV or BoHV-1 fused to the ORFs of a transcription-activating protein (B42) or a promoter-specific DNA-binding protein (LexA), respectively. Relevant restriction sites and retained amino acid sequences of the UL3.5 and UL48 proteins are shown in panels B and C. (D) Amino acid sequence of PrV pUL3.5. Peptides detected by MALDI-TOF analyses of the virion proteins are underlined, and identified features of different parts of the protein are indicated by shaded rectangles.