FIG. 3.

Two-hybrid interactions of pUL3.5 and pUL48. Yeast cells were cotransformed with plasmids expressing DNA-binding LexA and transcription-activating B42 fusion proteins (see Fig. 1C) and an inducible LacZ reporter plasmid to detect specific protein interactions. The empty vectors expressing B42 and LexA were used as controls, and the β-galactosidase activity of two clones per plasmid combination was assayed using ONPG as a substrate (Miller units/ml).