FIG. 3.

Negative correlation between the frequencies of HIV-2 Gag-specific IFN-γ⁺ or TNF-α⁺ CD4⁺ (A) and IFN-γ⁺ CD8⁺ (B) T cells with HIV-1 plasma viral load in dually infected subjects. A Spearman ranking test was used to analyze the correlation. Positive responses were designated when the percentage of bright cytokine⁺ CD4⁺ or CD8⁺ T cells was twice that of the negative control and >0.02% of the total CD4⁺ or CD8⁺ T cells. Antigen-specific responses of <0.01% are shown as 0.01% on the figures.