FIG. 2.

DC-target cell contact is required for efficient HIV transmission mediated by iDCs and mDCs. Transmission of HIV-Luc/JRFL with iDCs and mDCs as donors and (A) Hut/CCR5 cells or (B) GHOST/R5 cells as targets was performed as described in the legend to Fig. 1B. Transwell culture plates with membrane pore sizes of 3 μm were used to separate DCs and target cells (Transwell). HIV infection was determined after 2 dpi by measuring the luciferase activity. The data show the means ± standard deviations of triplicate samples. One representative experiment out of three is shown. cps, counts per second.