FIG. 5.

Effects of trafficking inhibitors on DC-mediated viral transmission. iDCs (A) and mDCs (B) were incubated separately with various inhibitors for 0.5 h and pulsed with HIV-Luc/JRFL in the presence of the inhibitors for 2 h at 37°C. DCs were washed and cocultured with Hut/CCR5 cells for 2.5 days. Medium that contained dissolvent was used as a control. The average relative transmission of four independent experiments using DCs from different donors is shown (medium controls were set at 100%). Asterisks indicate significant differences (*, P < 0.05; **, P < 0.01) compared with medium controls. (C) Viability of inhibitor-treated DCs after 3 days in culture. DCs were incubated separately with various inhibitors at 37°C for 2.5 h, washed, and cultured 3 days before staining with 7-amino-actinomycin D. Stained DCs were analyzed by flow cytometry. The average DC viability of three independent experiments is shown (medium controls were set at 100%).