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ELISA titration of anti-A33 MAbs. The wells of ELISA plates were coated with recombinant A33 or unrelated proteins (BSA, thyroglobulin, lysozyme, and phosphorylase b) and then incubated with serial dilutions of 6C, 12C, or 12F IgG. Bound IgG was detected by the addition of peroxidase-conjugated anti-human Fc followed by tetramethylbenzidine (TMB) substrate. Anti-A33 MAbs did not bind to the unrelated proteins (data not shown). OD450, optical density at 450 nm.
