FIG. 6.

Translocation of Arf to membranes induced by mutant 3CD proteins. RNAs coding for wt 3CD or for 3CDs with the indicated mutations in position 441 were translated in HeLa S10 extracts. The membranous fraction was collected by centrifugation and assessed by immunoblotting with anti-Arf antibodies (upper panel). The same membrane used for the Arf immunoblot was stripped and probed with antibodies to Rab9, as a loading control (middle panel). The lower panel shows the [³⁵S]methionine-labeled products of the translation reactions.