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Replication of poliovirus replicons harboring mutations that inhibit 3D elongation activity. Renilla luciferase poliovirus replicon RNA transcripts were transfected into HeLa cells growing in 96-well plates and incubated in the presence of cell-permeative Renilla luciferase substrate. Light readings were taken hourly with a Molecular Devices MV reader. RLU, relative light units. Error bars indicate standard deviations.
