TABLE 1.
3CD amino acid 441 (codon) | % of Arf activation by 3CDa | Full-length RNA specific infectivity | Position 441 reversion (codon) |
---|---|---|---|
F (TTC) (wt) | 100 | wt | NAb |
Y (TAC) | 86 | wt | NA |
I (ATT) | 71 | wt | NA |
L (TTA) | 60 | wt | NA |
S (TCC) | 9 | Quasi-infectious | F (TTC) |
S (TCA) | NDc | Quasi-infectious | L (TTA) |
A (GCA) | 35 | Quasi-infectious | F (TTC) |
R (AGA) | 4 | Quasi-infectious | I (ATA) |
E (GAA) | 1 | Quasi-infectious | V (GTA) |
The percentage of Arf activation was quantitated by analyzing the Arf immunoblot image with Total Lab 1D software.
NA, not applicable.
ND, not determined.