FIG. 3.

ICP4 domains required for B complex formation. (A) Schematic diagrams of WT ICP4 and different deletion mutants (drawn to scale). The numbers on the top indicate the amino acid positions. The map of the known domains of ICP4 (indicated by empty boxes) was adapted from references 14 and 50. (B) Results of an EMSA of mutant and WT ICP4 DNA complexes. Purified WT ICP4 and the mutant proteins were incubated with DNA probes derived from plasmids pk1-2 (lanes 2 to 6) and pBend4 (probe D) (lanes 8 to 12). Lanes 1 and 7 contained reaction mixtures lacking protein.