FIG. 6.
ICP4 contacts DNA outside its cognate binding site in the B complex. (A) Schematic diagram identifying the ICP4-DNA and n208-DNA complexes. (B) DNase I protection for the top strand of the probe by ICP4 and n208. (C) DNase I protection for the bottom strand of the probe by ICP4. A, ICP4-DNA A complex; A″, n208-DNA A complex; B, ICP4-DNA B complex; F, free DNA in ICP4-DNA binding reaction; F″, free DNA in n208-DNA binding reaction; M, CT- or G-specific sequencing ladder of the probe. The specific ICP4 binding site and extra DNA sequence (Seq.) protected are shown by vertical lines.