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RNA synthesis by wild-type EAV and SL5 mutants. Infectious RNA was transfected into BHK-21 cells, and intracellular RNA was isolated at 14 h posttransfection. The RNA was separated in a denaturing agarose gel and analyzed by hybridization to an oligonucleotide detecting all plus-stranded viral RNAs. The positions of the genome (RNA1) and sg mRNAs (RNA2 to RNA7) are indicated.
