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Viability of SL5 stem mutantsa
| Mutant | IFA at the indicated time points posttransfection
|
Titer (PFU/ml) at 24 h posttransfection | ||
|---|---|---|---|---|
| 14 h | 24 h | 40 h | ||
| wt | ++ | +++ | X | 9 × 106 |
| 1L | − | − | − | ND |
| 1R | − | − | − | ND |
| 1LR | ++ | +++ | X | 5 × 106 |
| 2L | − | − | − | ND |
| 2R | − | − | − | ND |
| 2LR | ++ | +++ | X | 3 × 106 |
| 3L | − | − | − | ND |
| 3R | − | − | − | ND |
| 3LR | + | ++ | +++ | 2 × 104 |
X, cells were detached and dying from infection. ND, not determined.
