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. 2007 Jun 20;81(17):9368–9376. doi: 10.1128/JVI.00427-07

FIG. 2.

FIG. 2.

Gene modulation by RT-PCR in E2-transfected HeLa cells compared to E6/E7 and E6AP siRNA-transfected cells. (A) Modulation of a panel of mitotic genes. HeLa cells were transfected with the E6/E7 siRNA cloned in a pSUPER expression vector or with a vector expressing HPV18 GFP-E2. RT-PCR values are given in arbitrary units (au) with the control siRNA set up to an arbitrary value of 1,000. (B) Modulation of p53 target genes. HeLa cells were transfected with siRNA against E6/E7 or E6AP, and the RT-PCR values of gene expression were compared to cells transfected by a control siRNA. Values given are calculated as in panel A. (C) Modulation of a panel of p63 target genes as in panel B. (D) Modulation of a panel of mitotic genes as in panel B.