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. 2007 Jun 20;81(17):9368–9376. doi: 10.1128/JVI.00427-07

TABLE 2.

Genes of cell cycle, cell proliferation, and motility repressed by E2 in the microarraysa

E2 target Name or description UniGene Hs cluster Function Repression (n-fold)
Mitosis
    ANLN Anillin 62180 Actin binding protein involved in cytokinesis 0.22
    BIRC5 Survivin 514527 Inhibitor of apoptosis; senses kinetochore tension 0.2
    BUB1 Homolog of mitotic checkpoint Saccharomyces erevisiae BUB1 469649 Spindle assembly checkpoint; linked to genetic instability 0.34
    CCNB1 Cyclin B1 23960 Complexes with p34 cdc2 to form the mitosis promoting factor 0.31
    Cdc25C Cell cycle division 656 M phase inducer phosphatase; activates cdc2 by dephosphorylation 0.36
    CDCA1 Cell division cycle associated 1 234545 Homolog of yeast Nuf2; component of the NDC80 kinetochore 0.27
    CDCA8 Cell division cycle associated 8 524571 Component of the chromosomal passenger complex 0.34
    CDKN2C Cyclin-dependent kinase Inhibitor 525324 P18/INK4 inhibits cyclin/cyclin-dependent kinase complexes 0.46
    CENPA Centromeric protein 1594 Component of modified nucleosome; interacts with Aurora A 0.26
    CENPF Centromeric protein 497741 Kinetochore 0.23
    CHK1 Checkpoint homolog 24529 Inhibits cdc25C; prevents activation of cyclinB/cdc2; DNA damage checkpoint 0.44
    CKS1 Cdc28 protein kinase 1B 374378 Activates cdc20 transcription; substrate targeting subunit of the SCF ubiquitin ligase 0.45
    CKS2 Cdc28 protein kinase 83758 Cdc2-associated protein (meiosis) 0.41
    DEPDC1B DEP domain-containing protein 1B 482233 DEP-containing protein 0.25
    DIAPH3 Diaphanous protein homolog 3 283127 Binds to Rho and to profilin; involved in cytokinesis 0.36
    ECT-2 Epithelial cell transforming 2 518299 Oncogen; binds to Rhoa protein to activate mitosis (ortholog of Drosophila Pebble) 0.33
    FBXO Emi1 F box only 520506 Interacts with cdc20 and inhibits the APC mitotic ubiquitin ligase 0.37
    HisH4F Histone H4F 247816 Forms centromers with CENPA 0.2
    HURP (DLG7) Hepatoma up-regulated 77695 Cell cycle regulator; up-regulated in G2/M 0.22
    KIF11 Kinesin family member 11 8878 Spindle assembly 0.25
    KIF22 Kinesin member 22 119324 Metaphase chromosome alignment 0.42
    KIF4A Kinesin family member 4 279766 Motor protein that translocates PRC1 0.33
    LBR Lamin B receptor 435166 Role in chromosome assembly 0.39
    MPHOSDH1 M phase phosphoprotein 240 Kinesin-related protein; M-phase promoting factor 0.25
    NUP188 Nucleoprotein 308340 0.34
    NUP107 Nucleoporin 524574 Nucleo-cytoplasmic trafficking; part of the nuclear pore and associated to the kinetochores 0.44
    NUSAP1 Nucleolar and spindle associated protein 1 511093 Involved in mitotic spindle organization 0.29
    PRC1 Protein regulating cytokinesis 567385 Associated with mitotic spindles; associates with KIF4; organizes spindle midzone formation 0.23
    RACGAP1 Rac GTPase activated protein 505469 Master regulator of initiation of cytokinesis 0.32
    SGOL1 Shugoshin-like 1 105153 Binds and stabilizes microtubules; localized to kinetochores 0.35
    SPBC25 Kinetochore 421956 Component of the NDC80 kinetochore complex 0.26
    SPC24 Spindle pole body 24 381225 Component of the NDC80 kinetochore complex 0.33
    STK6 Ser/Thr kinase 6 250822 Aurora A is targeted to the spindle apparatus by TPX2 and regulates its activity 0.34
    STMN1 Stathmin 209983 Sequesters tubulin in a ternary complex; role in depolymerization of mitotic microtubules 0.28
    TTK TTK protein kinase 169840 MPS1L1, PYT, and ESK; required for normal centrosome duplication 0.44
    TMPO Thymopoietin lamina-associated polypeptide 11355 Structural organization of the nucleus; postmitotic nuclear assembly 0.35
    TOPK Mitotic protein kinase 104741 Mitotic Ser/Thr kinase related to mitogen-activated protein kinase kinase 0.27
    TPX2 Targeting protein for Xklp2 244580 Proliferating associated protein P100; associated with spindle pole and spindle 0.30
Replication and DNA repair
    BARD1 BRCA1-associated ring domain 1 54089 Implicated in BRCA1 tumor suppression and DNA repair 0.36
    CCNE2 Cyclin E 567387 G1/S transition 0.1
    DCC1 RFC alternative complex 315167 Primed loading of PCNA on gapped DNA; defective sister chromatin cohesion 0.34
    DTYMK Thymidylate kinase 471873 DNA synthesis pathway catalyzes dMTP to dTDP 0.45
    HNRPA1 Heterogenous nuclear ribonucleopreotein 546261 Interacts with Fen1 during Okazaki fragment maturation 0.46
    MCM4 Minichromosome maintenance 460184 Replication licensing complex, loaded at the origins before initiation and essential for elongation 0.47
    MELK Maternal embryonic leucine zipper kinase 184339 Implicated in stem cell renewal and cell cycle progression 0.31
    NBS1 Nibrin 492208 Part of the DNA repair complex MRE11/RAD50 0.42
    Pfs2 GINS2 433180 DNA replication complex GINS 0.29
    PRIM2A Primase polypeptide A 485640 Polymerase that synthesizes small RNA primers (Okazaki) 0.45
    RFC3 Replication factor C 115474 DNA clamp loading complex; necessary for elongation of primed DNA 0.33
    TREX2 3′ Repair exonuclease 2 170835 DNA replication and DNA repair 0.47
    UNG Uracyl-DNA glycosylase 191334 DNA replication and DNA repair 0.3
    USP1 Ubiquitin specific protease 35086 Involved in DNA repair; Fanconi anemia pathway 0.4
Cell proliferation
    DHFR Dihydrofolate reductase 83765 Required for synthesis of purines, thymidylic acid, and certain amino acids 0.32
    DEK DEK oncogene 110713 Acute myeloma; oncogene 0.46
    DLEU2 Deleted in lymphocytic leukemia 508041 Candidate tumor suppressor gene 0.4
    HMMR Hyaluronan mediated motility receptor 72550 Involved in cell motility; may also be involved in cellular transformation and metastasis 0.22
    IGSF1 Immunoglobin superfamily 22111 Role in cell adhesion 0.4
    ITGB3BP Human beta 3 endonexin 1741 Coactivator of hormone receptors 0.44
    MLF1 Myeloid leukemia factor 1 85195 Oncoprotein; negative regulator of cell cycle 0.47
    NET1 Neuroepithelila cell transforming gene 25155 Rho exchange factor 0.44
    RHOG RAS homolog gene family G 501728 GTP-binding protein; activates RAC 0.48
    SHCBP1 Shc SH2 domain protein 123253 Linking activated cell surface receptors to the Ras pathway 0.32
    SHMT1 Serine hydroxy methyltransferase 1 513987 Plays an essential role in nucleic acids biosynthesis 0.24
    SHMT2 Serine hydroxy methyltransferase 2 75069 Cytosolic; mitochondrial 0.45
    SRC v-src sarcoma 195659 Protoncogene 0.24
    SYTL2 Synaptotagmin-like protein 369520 Binds to Rab27; involved in vesicle trafficking 0.42
    THBS2 Thrombospondin 371147 Inhibitor of tumor growth and angiogenesis 0.32
Miscellaneous
    ASF1B Anti-silencing function 26516 Assembles nucleosome during nucleotide excision repair 0.33
    BCAT1 Branched-chain amino transferase 438993 Direct target of c-Myc regulation 0.42
    CPSF1 Cleavage polyadenylation specific factor 493202 Addition of poly(A) tail to mRNA 0.43
    DDX39 DEAD box protein 39 311609 ATP-dependent RNA helicase 0.44
    JUNB JunB proto-oncogene 25292 Member of the AP1 transcription factor 0.41
    MNS1 Meiosis-specific nuclear structural protein 444483 Meiosis 0.42
    NF-YB Nuclear transcription factor Y 84928 Binding to the CCAAT motif 0.41
    RBL1 Retinoblastoma-like; P107 207745 Partner of E2F4/5 0.47
    SMYD3 Set and Mynd domain containing 3 567571 Histone methyl transferase 0.46
    SNRPB Splicing factor 83753 Knockout generates mitotic spindle defect; part of snRNP 0.44
a

Known E2F target genes are in bold. Numbers given in the last column are levels of transcription in E2-expressing cells compared to GFP-expressing cells; underlining indicates that the gene is present more than once in the array.