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Knockdown of Ero1-Lα expression in 3T3-L1 adipocytes inhibits secretion of adiponectin. 3T3-L1 adipocytes were transiently transfected with siRNAs corresponding to Ero1-Lα (1, 2, and 3) or a control siRNA as described in Materials and Methods. On day 6, total cell extracts (intracellular) and medium (extracellular) were harvested and subjected to Western blot analysis of Ero1-Lα, adiponectin, actin, and adipsin.
