FIG. 8.

Ectopic expression of Ero1-Lα enhances secretion of adiponectin in 3T3 adipocytes. (A) Swiss 3T3 fibroblasts expressing either WT-PPARγ (WT) or the mutant F372APPARγ (F) were induced to differentiate as described in Materials and Methods, and total cell extracts (Int) as well as medium (Ext) were harvested at day 5 for Western blot analysis of the indicated proteins. (B and C) Swiss-F372APPARγ cells expressing either a pREV-TET Ero1-Lα retrovirus (F-Ero) or a control vector (F-Con) were induced to differentiate in the presence (+) or absence (-) of tetracycline for 5 days as described in Materials and Methods. At this stage, medium (Ext) and total cell protein (Int) were harvested and subjected to reducing (B) or nonreducing without heat (C) SDS-PAGE followed by Western blot analysis using antibodies against the indicated proteins. In panel C, WT proteins from panel A were also analyzed as a control.